EMD Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA007633LA01HU
- Bilder (5)
| Artikelname: | EMD Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA007633LA01HU |
| Hersteller Artikelnummer: | CSB-PA007633LA01HU |
| Alternativnummer: | CSB-PA007633LA01HU-100UG, CSB-PA007633LA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | EDMD antibody, Emd antibody, EMD_HUMAN antibody, Emerin antibody, Emery Dreifuss muscular dystrophy antibody, STA antibody |
| Klonalität: | Polyclonal |
| UniProt: | P50402 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | EMD |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
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Immunofluorescent analysis of Hela cells using CSB-PA007633LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA007633LA01HU at dilution of 1:100 |
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Immunohistochemistry of paraffin-embedded human bladder cancer using CSB-PA007633LA01HU at dilution of 1:100 |
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Immunoprecipitating EMD in Hela whole cell lysate Lane 1: Rabbit ,control ,IgG ,instead ,of (1µg) instead of CSB-PA007633LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA007633LA01HU (8µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (10µg) |
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Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Colo320 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Mouse lung tissue All lanes: EMD antibody at 2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29 kDa Observed band size: 35 kDa |
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