EMC8 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA005835LA01HU
Artikelname: EMC8 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA005835LA01HU
Hersteller Artikelnummer: CSB-PA005835LA01HU
Alternativnummer: CSB-PA005835LA01HU-100UG, CSB-PA005835LA01HU-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: C16orf2 antibody, C16orf4 antibody, COX4 neighbor antibody, COX4, neighbor of antibody, COX4AL antibody, Cox4nb antibody, CX4NB_HUMAN antibody, ER membrane protein complex subunit 8 antibody, FAM158B antibody, family with sequence similarity 158, member B antibody, Neighbor of COX4 antibody, NOC4 antibody, Protein FAM158B antibody
Klonalität: Polyclonal
UniProt: O43402
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: >95%, Protein G purified
Formulierung: Liquid
Target-Kategorie: EMC8
Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA005835LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA005835LA01HU at dilution of 1:100
Western blot
All lanes: EMC8 antibody at 4µg/ml
Lane 1: A549 whole cell lysate
Lane 2: Human placenta tissue
Lane 3: Hela whole cell lysate
Lane 4: HT29 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 24, 14 kDa
Observed band size: 24 kDa
Immunofluorescence staining of MCF-7 cells with CSB-PA005835LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).