CD96 Antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: CSB-PA004971LA01HU
Artikelname: CD96 Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: CSB-PA004971LA01HU
Hersteller Artikelnummer: CSB-PA004971LA01HU
Alternativnummer: CSB-PA004971LA01HU-100UG, CSB-PA004971LA01HU-50UG
Hersteller: Cusabio
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, WB
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: CD96 antibody, CD96 molecule antibody, Cell surface antigen CD96 antibody, DKFZp667E2122 antibody, MGC22596 antibody, T cell activated increased late expression protein antibody, T cell activation, increased late expression antibody, T cell surface protein tactile precursor antibody, T cell-activated increased late expression protein antibody, T-cell surface protein tactile antibody, TACT_HUMAN antibody, Tactile antibody
Klonalität: Polyclonal
UniProt: P40200
Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Reinheit: Antigen Affinity Purified
Formulierung: Liquid
Target-Kategorie: CD96
Application Verdünnung: Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200,
Immunofluorescence staining of HepG2 cell with CSB-PA004971LA01HU at 1:110, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: MCF7 whole cell lysate(20µg), 293 whole cell lysate(20µg), JK whole cell lysate(20µg), K562 whole cell lysate(20µg), Hela whole cell lysate(20µg)
All lanes: CD96 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 66,100-200kd kDa
Observed band size: 100 kDa
Exposure time: 60s