CAST Antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:
CSB-PA004561YA01HU
- Bilder (4)
| Artikelname: | CAST Antibody, Unconjugated, Rabbit, Polyclonal |
| Artikelnummer: | CSB-PA004561YA01HU |
| Hersteller Artikelnummer: | CSB-PA004561YA01HU |
| Alternativnummer: | CSB-PA004561YA01HU-100UG, CSB-PA004561YA01HU-50UG |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC, WB |
| Spezies Reaktivität: | Human, Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BS 17 antibody, Calpain inhibitor antibody, Calpastatin antibody, Cast antibody, Heart type calpastatin antibody, ICAL_HUMAN antibody, MGC9402 antibody, Sperm BS 17 component antibody, Sperm BS-17 component antibody |
| Klonalität: | Polyclonal |
| UniProt: | P20810 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | CAST |
| Application Verdünnung: | Recommended dilution: WB:1:1000-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
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Immunofluorescence staining of A549 cells with CSB-PA004561YA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA004561YA01HU diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA004561YA01HU diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: HL60 whole cell lysate, Mouse heart tissue All lanes: CAST antibody at 2.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 76, 64, 72, 82, 85, 83, 75, 81 kDa Observed band size: 77 kDa |
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