Immunofluorescence staining of A549 cells with CSB-PA00384A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00384A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA00384A0Rb at dilution of 1:100
Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, 293 whole cell lysate All lanes: EIF4A2 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa
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