Gephyrin/GPHN Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB763172
Artikelname: Gephyrin/GPHN Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB763172
Hersteller Artikelnummer: orb763172
Alternativnummer: BYT-ORB763172-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, ICC, IF, IHC, WB
Spezies Reaktivität: Human, Monkey, Mouse, Rat
Immunogen: E.coli-derived human Gephyrin/GPHN recombinant protein (Position: K148-L736).
Konjugation: Unconjugated
Alternative Synonym: Domain E, GEPH, gephyrin, GPH, GPHN, GPHRYN, KIAA1385, MPT adenylyltransferase, MPT Mo transferase
Anti-Gephyrin/GPHN Antibody. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Klonalität: Polyclonal
Konzentration: 500 µg/ml
Molekulargewicht: 93 kDa
UniProt: Q9NQX3
Puffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Formulierung: Lyophilized
Target-Kategorie: Gephyrin [Includes: Molybdopterin adenylyltransferase
Application Verdünnung: Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, -
IF analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Gephyrin/GPHN Antibody overnight at 4C. DyLight594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Gephyrin/GPHN Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in a paraffin-embedded section of human papillary thyroid carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Gephyrin/GPHN Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Gephyrin/GPHN Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Gephyrin/GPHN Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Gephyrin/GPHN Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody. Gephyrin/GPHN was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then i