Human Collagen Type IV protein, Unconjugated
Artikelnummer:
BYT-ORB750384
- Bilder (4)
| Artikelname: | Human Collagen Type IV protein, Unconjugated |
| Artikelnummer: | BYT-ORB750384 |
| Hersteller Artikelnummer: | orb750384 |
| Alternativnummer: | BYT-ORB750384-500 |
| Hersteller: | Biorbyt |
| Kategorie: | Proteine/Peptide |
| Applikation: | ELISA, IHC, SDS-PAGE, WB |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Type IV collagen protein, collagen 4 protein, human collagen protein, Collagen alpha-4 (IV) chain protein |
| Human Collagen Type IV protein |
| Konzentration: | 1.0 mg/ml |
| UniProt: | P02462 |
| Puffer: | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None, Buffer: 0.5 M Acetic Acid |
| Quelle: | Human |
| Reinheit: | This product has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA). |
| Formulierung: | Liquid (sterile filtered) |
| Target-Kategorie: | COL4A1-COL4A6 |
| Application Verdünnung: | IF: 1:100-1:500, WB: 1:1000 |
| Anwendungsbeschreibung: | Biological Origin: Human. Application Notes: For use as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user |
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Knockdown of NORAD inhibits proliferation, inflammation and fibrosis in HG-induced HMCs. Levels of (G) Col. IV in HMCs measured via ELISA. **P < 0.01 vs. NG, P < 0.01 vs. HG + si-NC. NORAD, non-coding RNA activated by DNA damage, NG, normal glucose, HG, high glucose, HMCs, human mesangial cells, si, small interfering RNA, NC, negative control Col. IV, type IV collagen. |
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NORAD knockdown inhibits proliferation, inflammation and fibrosis in HG-induced HMCs by regulating the miR-485/NRF1 axis. (G) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs as determined via ELISA. **P < 0.01 vs. si-NC, P < 0.05 vs. si-NORAD + inhibitor NC, &P < 0.05 vs. si-NORAD + ov-NC. miR, microRNA, NORAD, non-coding RNA activated by DNA damage, NRF1, nuclear respiratory factor 1, HG, high glucose, HMCs, human mesangial cells, ov, overexpression vector, si, small interfering RNA, NC, negative control, Col. IV, type IV collagen, FN, fibronectin, PAI-1, plasminogen activator inhibitor 1. |
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NORAD reverses the inhibitory effects of miR-485 on HG-induced HMCs. (F) Contents of Col. IV, FN and PAI-1 in HG-induced HMCs measured via ELISA **P < 0.01 vs. miR-NC, P < 0.05 vs. miR-485 mimics. miR, microRNA, NORAD, non-coding RNA activated by DNA damage, HG, high glucose, HMCs, human mesangial cells, ov, overexpression vector, NC, negative control, Col. IV, type IV collagen, FN, fibronectin, PAI-1, plasminogen activator inhibitor 1. |
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SDS-PAGE Results of Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5.0 µg). Lane 2: Opal Prestained Molecular Weight Protein. Lane 3: Human Collagen Type IV Non-Reduced (5.0 µg). 4-20% Gel, Coomassie Stained. Observed MW: Type IV alpha1, alpha2, alpha3 bands. |
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