SIRT3 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB704396
- Bilder (6)
| Artikelname: | SIRT3 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB704396 |
| Hersteller Artikelnummer: | orb704396 |
| Alternativnummer: | BYT-ORB704396-100, BYT-ORB704396-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human SIRT3 (300-399/399aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | SIR2L3, 2310003L23Rik, SIR3_HUMAN, SIRT3, hSIRT3, NAD-dependent protein delactylase sirtuin-3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, 2.3.1.286, SIR3_MOUSE, SIR2-like protein 3 (mSIR2L3), |
| SIRT3 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 28/43 kDa |
| UniProt: | Q9NTG7 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SIRT3 |
| Application Verdünnung: | IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
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NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (KO Validated) SIRT3 polyclonal Antibody, Unconjugated (orb704396) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human thyroid cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (orb704396) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (orb704396) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (orb704396) at 1:100 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: 293T (Human) Cell Lysate at 25 ug, Lane 2: SIRT3 knockout (KO) 293T (Human) Cell Lysate at 25 ug, Primary: Anti-SIRT3 (orb704396) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 28 kD, Observed band size: 30 kD. |
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Sample: Lane 1: Panc-1 (Human) Cell Lysate at 30 ug, Lane 2: 293T (Human) Cell Lysate at 30 ug, Lane 3: K562 (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-SIRT3 (orb704396) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD. |
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