Beclin 1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB704275
- Bilder (7)
| Artikelname: | Beclin 1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB704275 |
| Hersteller Artikelnummer: | orb704275 |
| Alternativnummer: | BYT-ORB704275-50,BYT-ORB704275-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human BECN1 (181-280/450aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ATG6, VPS30, beclin1, BECN1_HUMAN, BECN1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, GT197, BECN1_MOUSE, BECN1_RAT, |
| Beclin 1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 60 kDa |
| UniProt: | Q14457 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | BECN1 |
| Application Verdünnung: | WB=1:500-1000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (orb704275) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Small intestine (Mouse) Lysate at 40 ug, Lane 3: Lung (Mouse) Lysate at 40 ug, Lane 4: Heart (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Lane 6: Jurkat (Human) Cell Lysate at 30 ug, Lane 7: Raji (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: A431 (Human) Cell Lysate at 30 ug, Lane 10: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Beclin 1 (orb704275) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55-60 kD, Observed band size: 60 kD. |
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IHC-P analysis of mouse brain using Beclin 1 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of rat brain using Beclin 1 Polyclonal Antibody at 1:200 dilution. |
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