P70 S6 Kinase Beta Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6616
- Bilder (6)
| Artikelname: | P70 S6 Kinase Beta Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6616 |
| Hersteller Artikelnummer: | orb6616 |
| Alternativnummer: | BYT-ORB6616-50,BYT-ORB6616-100,BYT-ORB6616-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human RPS6KB2 (165-270/482aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | KLS, P70-beta, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, S6KB, S6KI(2), S6Kbeta, SRK, STK14B, p70(S6K)-beta, p70S6Kb, S6K-beta-2, KS6B2_HUMAN, RPS6KB2, 70 kDa ribosomal protein S6 kinase 2 (P70S6K2 | p70-S6K 2), S6 kinase-related kinase (SRK), Serine/threonine-protein kinase 14B, p70 ribosomal S6 kinase beta (S6K-beta | p70 S6 kinase beta | p70 S6K-beta | p70 S6KB | p70-beta), 2.7.11.1, KS6B2_MOUSE, 70 kDa ribosomal protein S6 kinase 2, p70 ribosomal S6 kinase beta (p70 S6 kinase beta | p70 S6K-beta | p70 S6KB), |
| P70 S6 Kinase Beta Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 53 kDa |
| UniProt: | Q9UBS0 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | RPS6KB2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
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Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-p70 S6 Kinase Beta antibody (orb6616), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (p70 S6 Kinase Beta) polyclonal Antibody, Unconjugated (orb6616) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (p70 S6 Kinase Beta) Polyclonal Antibody, Unconjugated (orb6616) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p70 S6 Kinase Beta) Polyclonal Antibody, Unconjugated (orb6616) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (p70 S6 Kinase Beta) Polyclonal Antibody, Unconjugated (orb6616) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human Jurkat cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HeLa cell lysates, Primary: Anti-p70 S6 Kinase Beta (orb6616) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kDa, Observed band size: 58 kDa. |
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