Phospho-RPS6KB1 (Thr412) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6611
- Bilder (6)
| Artikelname: | Phospho-RPS6KB1 (Thr412) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6611 |
| Hersteller Artikelnummer: | orb6611 |
| Alternativnummer: | BYT-ORB6611-50,BYT-ORB6611-100,BYT-ORB6611-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human RPS6KB1 around the phosphorylation site of Thr412 GF(p-T)YV |
| Konjugation: | Unconjugated |
| Alternative Synonym: | RPS6KB1 | p70-S6K1 (p-T412), p-p70-S6K1, phospho-p70-S6K1, PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha, 2610318I15Rik, P70S6K1, p70 S6K-alpha, p70-S6K 1, p70/85s6k, p70s6k, KS6B1_HUMAN, RPS6KB1, 70 kDa ribosomal protein S6 kinase 1 (P70S6K1 | p70-S6K 1), Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha (p70 S6 kinase alpha | p70 S6K-alpha | p70 S6KA), 2.7.11.1, KS6B1_MOUSE, Ribosomal protein S6 kinase I (S6K), KS6B1_RAT, |
| Phospho-RPS6KB1 (Thr412) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 58 kDa |
| UniProt: | P23443 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | RPS6KB1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RPS6KB1 (Thr412)) Polyclonal Antibody, Unconjugated (orb6611) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RPS6KB1 (Thr412)) Polyclonal Antibody, Unconjugated (orb6611) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RPS6KB1 (Thr412)) Polyclonal Antibody, Unconjugated (orb6611) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-RPS6KB1 (Thr412) (orb6611) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD. |
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Immunohistochemical staining of Rat Thyroid tissue using RPS6KB1 (phospho-Ser411) antibody. |
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Immunohistochemical staining of rat brain tissue using RPS6KB1 (phospho-Ser411) antibody. |
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