JNK2/MAPK9 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6256
- Bilder (6)
| Artikelname: | JNK2/MAPK9 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6256 |
| Hersteller Artikelnummer: | orb6256 |
| Alternativnummer: | BYT-ORB6256-100,BYT-ORB6256-200,BYT-ORB6256-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human JNK2 (311-424/424aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK, MK09_HUMAN, MAPK9, MAP kinase 9, MAPK 9, Stress-activated protein kinase 1a (SAPK1a), Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, 2.7.11.24, MK09_MOUSE, MK09_RAT, SAPK-alpha, p54-alpha, |
| JNK2/MAPK9 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 48 kDa |
| UniProt: | P45984 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | MAPK9 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK2) Polyclonal Antibody, Unconjugated (orb6256) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (JNK2) Polyclonal Antibody, Unconjugated (orb6256) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-JNK2 (orb6256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 50 kD. |
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Sample: Hela (Human) Cell Lysate at 30 ug, Uterus (Mouse) Lysate at 40 ug, Primary: Anti-JNK2 (orb6256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD. |
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Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Heart tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-JNK2/MAPK9 (orb6256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 50 kDa. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-JNK2 Polyclonal Antibody, Unconjugated (orb6256) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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