ATP5H in Mouse Monoclonal Antibody, Clone: [6B12], Unconjugated
Artikelnummer:
BYT-ORB623780
Hersteller Artikelnummer:
orb623780
Alternativnummer:
BYT-ORB623780-100
Hersteller:
Biorbyt
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ICC, IF, IHC, WB
Spezies Reaktivität:
Human, Monkey, Mouse, Rat
Immunogen:
E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.
Konjugation:
Unconjugated
Alternative Synonym:
ATP synthase D chain mitochondrial antibody, ATP synthase H+ transporting mitochondrial F1F0 subunit antibody, ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody, ATP synthase subunit d antibody, ATP synthase subunit d, mitochondrial antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody, ATP5H antibody, ATP5H_HUMAN antibody, ATP5JD antibody, ATPase subunit d antibody, ATPQ antibody, mitochondrial antibody, My032 protein antibody
Anti-ATP5H in Antibody (monoclonal, 6B12). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
ATP synthase peripheral stalk subunit d, mitochondrial
Application Verdünnung:
Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human Immunocytochemistry/Immunofluorescence, 2µg/ml, Human
IF analysis of ATP5H using anti-ATP5H antibody. ATP5H was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL mouse anti-ATP5H Antibody overnight at 4C. Cy3 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of ATP5H using anti-ATP5H antibody. ATP5H was detected in paraffin-embedded section of human B lymphocytic tumorT tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-ATP5H Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of ATP5H using anti-ATP5H antibody.
IHC analysis of ATP5H using anti-ATP5H antibody. ATP5H was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml mouse anti-ATP5H Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IF analysis of ATP5H using anti-ATP5H antibody.
Western blot analysis of ATP5H using anti-ATP5H antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human SW620 whole cell lysates. Lane 7: rat PC-12 whole cell lysates. Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ATP5H antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22 KD. The expected band size for ATP5H is at 18 KD.
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