TNMD Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB612264
Artikelname: TNMD Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB612264
Hersteller Artikelnummer: orb612264
Alternativnummer: BYT-ORB612264-50,BYT-ORB612264-100,BYT-ORB612264-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Recombinant human TNMD protein
Konjugation: Unconjugated
Alternative Synonym: BRICD4, CHM1L, TEM, 1110017I01Rik, TNMD_HUMAN, TNMD, hTeM, Chondromodulin-1-like protein (ChM1L | hChM1L), Chondromodulin-I-like protein, Myodulin, Tendin, TNMD_MOUSE, mTeM, Chondromodulin-1-like protein (ChM1L | mChM1L), TNMD_RAT, rTeM, Chondromodulin-1-like protein (ChM1L | rChM1L),
TNMD Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 37 kDa
UniProt: Q9H2S6
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: TNMD
Application Verdünnung: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb612264) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TNMD) Polyclonal Antibody, Unconjugated (orb612264) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Muscle (Rat) Lysate at 40 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Lane 4: SW480 (Human) Cell Lysate at 30 ug, Primary: Anti-TNMD (orb612264) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 35 kD.
Sample: Lane 1: Skeletal muscle (Rat) Lysate at 40 ug, Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug, Primary: Anti-TNMD (orb612264) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kD, Observed band size: 37 kD.
IHC-P analysis of rat heart using TNMD Polyclonal Antibody at 1:2000 dilution.
IHC-P analysis of human breast using TNMD Polyclonal Antibody at 1:2000 dilution.



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