PKC delta Recombinant Rabbit Monoclonal Antibody, Clone: [2C4], Unconjugated

Artikelnummer: BYT-ORB612223

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Artikelname:	PKC delta Recombinant Rabbit Monoclonal Antibody, Clone: [2C4], Unconjugated
Artikelnummer:	BYT-ORB612223
Hersteller Artikelnummer:	orb612223
Alternativnummer:	BYT-ORB612223-50,BYT-ORB612223-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	FC, ICC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human, Mouse, Rat
Immunogen:	KLH conjugated synthetic peptide derived from human PKC delta
Konjugation:	Unconjugated
Alternative Synonym:	ALPS3, CVID9, MAY1, PKCD, nPKC-delta, D14Ertd420e, PKC[d], PKCdelta, KPCD_HUMAN, PRKCD, Tyrosine-protein kinase PRKCD, 2.7.11.13, KPCD_MOUSE, KPCD_RAT,

PKC delta Recombinant Rabbit Monoclonal Antibody

Klonalität:	Recombinant
Konzentration:	1mg/ml
Klon-Bezeichnung:	[2C4]
Molekulargewicht:	77 kDa
UniProt:	Q05655
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	PRKCD

Application Verdünnung:

WB=1:1000-2000, IHC-P=1:100-200, IHC-F=1:100-200, ICC/IF=1:100-200, IF=1:100-200, Flow-Cyt=1:50-100

	Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PKC delta) Monoclonal Antibody, Unconjugated (orb612223) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PKC delta) Monoclonal Antibody, Unconjugated (orb612223) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PKC delta) Monoclonal Antibody, Unconjugated (orb612223) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Jurkat (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-PKC delta (orb612223) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 75 kD.
	IHC-P analysis of mouse lymphoid using PKC delta Monoclonal Antibody at 1:200 dilution.
	IHC-P analysis of rat cerebellum using PKC delta Monoclonal Antibody at 1:200 dilution.

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