CDKN2A/p16-INK4a Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB608177
Artikelname: CDKN2A/p16-INK4a Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB608177
Hersteller Artikelnummer: orb608177
Alternativnummer: BYT-ORB608177-50,BYT-ORB608177-100,BYT-ORB608177-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CDKN2A/p16-INK4a (61-156/156aa)
Konjugation: Unconjugated
Alternative Synonym: ARF, CAI2, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16, ARF-INK4a, INK4a-ARF, Ink4a/Arf, Pctr1, p16(INK4a), p19, p16Cdkn2a, CDN2A_HUMAN, CDKN2A, Cyclin-dependent kinase 4 inhibitor A (CDK4I), Multiple tumor suppressor 1 (MTS-1), p16-INK4a (p16-INK4 | p16INK4A), CDN2A_MOUSE, p16-INK4a (p16-INK4), CDN2A_RAT, p16-INK4a (p16 | p16-INK4),
CDKN2A/p16-INK4a Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 17 kDa
UniProt: P42771
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: Cdkn2a
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDKN2A/p16-INK4a) Polyclonal Antibody, Unconjugated (orb608177) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDKN2A) Polyclonal Antibody, Unconjugated (orb608177) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CDKN2A) Polyclonal Antibody, Unconjugated (orb608177) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-CDKN2A/p16-INK4a (orb608177) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 17 kD.
IHC-P analysis of mouse brain using CDKN2A Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of rat brain using CDKN2A Polyclonal Antibody at 1:200 dilution.