STAT3 Mouse Monoclonal Antibody, Clone: [5H4], Unconjugated
Artikelnummer:
BYT-ORB560168
- Bilder (6)
| Artikelname: | STAT3 Mouse Monoclonal Antibody, Clone: [5H4], Unconjugated |
| Artikelnummer: | BYT-ORB560168 |
| Hersteller Artikelnummer: | orb560168 |
| Alternativnummer: | BYT-ORB560168-50,BYT-ORB560168-100,BYT-ORB560168-200 |
| Hersteller: | Biorbyt |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant human STAT3 Protein (9-265/770aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ADMIO, ADMIO1, APRF, HIES, 1110034C02Rik, STAT3_HUMAN, STAT3, Acute-phase response factor, STAT3_MOUSE, STAT3_RAT, |
| STAT3 Mouse Monoclonal Antibody |
| Klonalität: | Monoclonal |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [5H4] |
| Molekulargewicht: | 88 kDa |
| UniProt: | P40763 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | STAT3 |
| Application Verdünnung: | WB=1:1000-5000, IHC-P=1:200-2000, IHC-F=1:200-2000, IF=1:200-2000 |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (orb560168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (STAT3) Monoclonal Antibody, Unconjugated (orb560168) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Raw264.7 cell lysates, Lane 3: Human HepG2 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human SW480 cell lysates, Lane 6: Human THP-1 cell lysates, Primary: Anti-STAT3 (orb560168) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 88 kDa, Observed band size: 92 kDa. |
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Sample: Lane 1: STAT3 knockout (KO) A549 Cell Lysate, Lane 2: Human A549 Cell (Control) Lysate, Primary: Anti-STAT3 (orb560168) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 88 kD. |
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Western blot:Heart (Mouse),Placenta (Mouse),Lung (Mouse),Primary:Anti-STAT3 at 1:1000 dilution. |
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