ULBP1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB5600
Artikelname: ULBP1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB5600
Hersteller Artikelnummer: orb5600
Alternativnummer: BYT-ORB5600-50,BYT-ORB5600-100,BYT-ORB5600-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ULBP1 (121-220/244aa)
Konjugation: Unconjugated
Alternative Synonym: N2DL-1, NKG2DL1, RAET1I, ULBP1_HUMAN, ULBP1, ALCAN-beta, NKG2D ligand 1 (N2DL-1 | NKG2DL1), Retinoic acid early transcript 1I, N2DL1,
ULBP1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 25 kDa
UniProt: Q9BZM6
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: ULBP1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ULBP1) Polyclonal Antibody, Unconjugated (orb5600) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ULBP1) Polyclonal Antibody, Unconjugated (orb5600) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ULBP1) Polyclonal Antibody, Unconjugated (orb5600) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ULBP1) Polyclonal Antibody, Unconjugated (orb5600) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat lymph), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ULBP1) Polyclonal Antibody, Unconjugated (orb5600) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Protein: rat kidney lysates, 30 ug, Primary: Anti-ULBP1 (orb5600) at 1:200, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 25kD, Observed band size: 43kD.