Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody, Clone: [1C6], Unconjugated
Artikelnummer:
BYT-ORB559154
- Bilder (6)
| Artikelname: | Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody, Clone: [1C6], Unconjugated |
| Artikelnummer: | BYT-ORB559154 |
| Hersteller Artikelnummer: | orb559154 |
| Alternativnummer: | BYT-ORB559154-50,BYT-ORB559154-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised acetylpeptide derived from human Histone H3 around the acetylation site of K14 |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H3C1 | Histone H3 (ac-K14), ac-Histone H3, acetyl-Histone H3, H3K14Ac, ac-H3, H3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, H3/b, H3C1, H3FB, HIST1H3D, H3.1, H3/c, H3FC, HIST1H3C, H3/d, H3FD, HIST1H3E, H3.f, H3/f, H3FF, HIST1H3I, H3/h, H3FH, HIST1H3G, H3/j, H3FJ, HIST1H3J, H3/k, H3F1K, H3FK, HIST1H3H, H3/l, H3FL, HIST1H3B, H3/i, H3FI, HIST1H3F, H3.2-221, H3c13, H3c14, H3c15, Hist1, H3-143, H3-b, H3-53, H3-f, H3-291, H3-H, H3.1-221, H3 histone, Hist1h2ail, Hist1h2ail1, Hist2h3c, Hist3h3, H31_HUMAN, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3FC HIST1H3C, H33_HUMAN, H3-3A, H3-3B, H3.3A, H3F3, H3F3A, H3.3B, H3F3B, H31T_HUMAN, H3-4, H3/t, H3t, H3/g, Histone H3.4, H3FT, H3C_HUMAN, H3-5, Histone H3.5, H3F3C, H32_HUMAN, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, Histone H3/m, Histone H3/o, HIST2H3A, H3F2, H3FM, HIST2H3D, H31_MOUSE, H3a, H3g, H3.1-291, H3h, H3.1-I, H3i, H32_MOUSE, H3-clustered histone 2, H3-clustered histone 3, H3-clustered histone 4, H3-clustered histone 6, H3-clustered histone 7, H3.2, H3b, H3f, H3.2-616, Hist2h3b, H3.2-615, Hist2h3c1, Hist2h3ca1, H3.2-614, Hist2h3c2, Hist2h3ca2, H31_RAT, |
| Histone H3 (acetyl K14) Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [1C6] |
| Molekulargewicht: | 15 kDa |
| UniProt: | P68431 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | H3C1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100 |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Histone H3 (acetyl K14)) monoclonal Antibody, Unconjugated (orb559154) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Histone H3 (acetyl K14)) monoclonal Antibody, Unconjugated (orb559154) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (acetyl K14)) Monoclonal Antibody, Unconjugated (orb559154) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3 (acetyl K14)) Monoclonal Antibody, Unconjugated (orb559154) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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PC-3M cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Histone H3 (acetyl K14)) monoclonal Antibody, Unconjugated (orb559154) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Sample: Lane 1: Jurkat cell lysate, Lane 2: rat liver tissue lysate, Lane 3: mouse lung tissue lysate, Primary: Anti-Histone H3 (acetyl K14) (orb559154) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 15 kD, Observed band size: 14 kD. |
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