IRF6 Recombinant Rabbit Monoclonal Antibody, Clone: [1A1], Unconjugated

Artikelnummer: BYT-ORB559090
Artikelname: IRF6 Recombinant Rabbit Monoclonal Antibody, Clone: [1A1], Unconjugated
Artikelnummer: BYT-ORB559090
Hersteller Artikelnummer: orb559090
Alternativnummer: BYT-ORB559090-50,BYT-ORB559090-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Recombinant human IRF6 protein, around C-terminal 150aa
Konjugation: Unconjugated
Alternative Synonym: LPS, OFC6, PIT, PPS, PPS1, VWS, VWS1, E230028I05Rik, mirf6, IRF6_HUMAN, IRF6, IRF-6, IRF6_MOUSE,
IRF6 Recombinant Rabbit Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1mg/ml
Klon-Bezeichnung: [1A1]
Molekulargewicht: 53 kDa
UniProt: O14896
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: IRF6
Application Verdünnung: WB=1:1000-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200
Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human K562 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human SW480 cell lysates, Lane 4: Human A431 cell lysates, Lane 5: Human SH-SY5Y cell lysates, Primary: Anti-IRF6 (orb559090) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kDa, Observed band size: 58 kDa.
IHC-P analysis of human gastric carcinoma using IRF6 Monoclonal Antibody at 1:200 dilution.