Phospho-IGF1R (Tyr980) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB5503
Artikelname: Phospho-IGF1R (Tyr980) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB5503
Hersteller Artikelnummer: orb5503
Alternativnummer: BYT-ORB5503-50,BYT-ORB5503-100,BYT-ORB5503-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980 PE(p-Y)FS
Konjugation: Unconjugated
Alternative Synonym: IGF1R (p-Y980), p-IGF1R, phospho-IGF1R, CD221, IGFIR, IGFR, JTK13, A330103N21Rik, D930020L01, IGF-1R, hyft, IGF-1 receptor, IGFIRC, Igfr1, IGF1R_HUMAN, IGF1R, Insulin-like growth factor I receptor (IGF-I receptor), 2.7.10.1, IGF1R_MOUSE, IGF1R_RAT,
Phospho-IGF1R (Tyr980) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 150 kDa
UniProt: P08069
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: IGF1R
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Anwendungsbeschreibung: Modification: Phosphorylated
Flow cytometric analysis of Mouse Kidney tissue using IGF1R (phospho-Tyr980) antibody.
Immunohistochemical staining of human kidney carcinoma tissue using IGF1R (phospho-Tyr980) antibody.
Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37C). Primary Antibody: Rabbit Anti-phospho-IGF1R (Tyr980) antibody (orb5503, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.
Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-phospho-IGF1R (Tyr980) antibody (orb5503), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RELM beta) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
The blue histogram is unstained cells (Hepg2 cells). The green histogram is cells stained with Rabbit Anti-phospho-IGF1R (Tyr980) antibody (orb5503) plus secondary antibody (orb868805).Concentration 1:20.
Tissue/Cell: human kidney carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-IGF1R (Tyr980) Polyclonal Antibody, Unconjugated (orb5503) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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