Bax Mouse Monoclonal Antibody, IgG1, Clone: [3C1], Unconjugated
Artikelnummer:
BYT-ORB500966
- Bilder (6)
| Artikelname: | Bax Mouse Monoclonal Antibody, IgG1, Clone: [3C1], Unconjugated |
| Artikelnummer: | BYT-ORB500966 |
| Hersteller Artikelnummer: | orb500966 |
| Alternativnummer: | BYT-ORB500966-50,BYT-ORB500966-100,BYT-ORB500966-200 |
| Hersteller: | Biorbyt |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ICC, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human Bax (31-130/192aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BCL2L4, BAX_HUMAN, BAX, Bcl-2-like protein 4 (Bcl2-L-4), BAX_MOUSE, BAX_RAT, |
| Bax Mouse Monoclonal Antibody |
| Klonalität: | Monoclonal |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [3C1] |
| Molekulargewicht: | 21 kDa |
| Isotyp: | IgG1 |
| UniProt: | Q07812 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | BAX |
| Application Verdünnung: | WB=1:500-2000, ICC/IF=1:50-200 |
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500966) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Mouse IgG antibody for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bax) Monoclonal Antibody, Unconjugated (orb500966) at 1:400 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: 293T (Human) Cell Lysate at 30 ug, 293T-UV (Human) Cell Lysate at 30 ug, Primary: Anti-Bax (orb500966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 21 kD. |
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Sample: Lane 1: Human 293T cell lysates, Lane 2: Human HT-1080 cell lysates, Lane 3: Human Raji cell lysates, Lane 4: Human SH-SY5Y cell lysates, Primary: Anti-Bax (orb500966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 20 kDa. |
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Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (orb500966) 1:100, 90 minutes at 37C, followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Western blot:Hela(Human) Cell,MCF-7(Human) Cell,Primary:Anti-Bax at 1:1000 dilution. |
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