0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
CD274
Application Verdünnung:
WB=1:500-2000
Western blot analysis of Mouse Lymph node Lysate using PD-L1 antibody.
Immunohistochemical staining of Mouse placenta tissue using PD-L1 antibody.
Western blot analysis of Mouse Spleen Lysate using PD-L1 antibody.
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-PD-L1 antibody (orb500797), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Sample: Lane 1: Recombinant mouse PD-L1 protein, C-His (HEK293), Primary: Anti-PD-L1 (orb500797) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 45 kDa.
Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD274 (orb500797) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 32 kD, Observed band size: 48 kD.
Tissue/Cell: Mouse placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-CD274 Polyclonal Antibody, Unconjugated (orb500797) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
