Sample: jurkat (human) cell Lysate at 40 ug, Primary: Anti-APOBEC3B (orb500784) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46kD, Observed band size: 46 kD.
Tissue/Cell: Mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (orb500784) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat ovary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (orb500784) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-APOBEC3B Polyclonal Antibody, Unconjugated (orb500784) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
IHC-P image of Rat ovary tissue using APOBEC3B antibody
IHC-P image of rat spleen tissue using APOBEC3B antibody
IHC-P image of Mouse spleen tissue using APOBEC3B antibody
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