Myoglobin Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB500695
Artikelname: Myoglobin Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB500695
Hersteller Artikelnummer: orb500695
Alternativnummer: BYT-ORB500695-50,BYT-ORB500695-100,BYT-ORB500695-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: Purified Human Myoglobin (Natural Human Myoglobin protein)
Konjugation: Unconjugated
Alternative Synonym: MYOSB, PVALB, Glng, MYG_HUMAN, MB, Nitrite reductase MB, Pseudoperoxidase MB, MYG_MOUSE, MYG_RAT,
Myoglobin Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 17 kDa
UniProt: P02144
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: MB
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (orb500695) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Myoglobin) Polyclonal Antibody, Unconjugated (orb500695) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Human Myoglobin Protein at 100 ng, Muscle (Mouse) Lysate at 30 ug, Muscle (Rat) Lysate at 20 ug, Primary: Anti-Myoglobin (orb500695) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 14 kD.
Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Heart (Rat) Lysate at 40 ug, Primary: Anti-Myoglobin (orb500695) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 17 kD.
IHC-P analysis of Human liver carcinoma using Myoglobin Polyclonal Antibody at 1:500 dilution.
IHC-P analysis of Rat skeletal muscle using Myoglobin Polyclonal Antibody at 1:400 dilution.



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