S100B Mouse Monoclonal Antibody, Clone: [1C6], Unconjugated
Artikelnummer:
BYT-ORB500644
- Bilder (9)
| Artikelname: | S100B Mouse Monoclonal Antibody, Clone: [1C6], Unconjugated |
| Artikelnummer: | BYT-ORB500644 |
| Hersteller Artikelnummer: | orb500644 |
| Alternativnummer: | BYT-ORB500644-50,BYT-ORB500644-100,BYT-ORB500644-200 |
| Hersteller: | Biorbyt |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant human S100B protein (2-92/92aa (N-6x His-Tag)aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | NEF, S100, S100-B, S100beta, S100B_HUMAN, S100B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, |
| S100B Mouse Monoclonal Antibody |
| Klonalität: | Monoclonal |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [1C6] |
| Molekulargewicht: | 10 kDa |
| UniProt: | P04271 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | S100B |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
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(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human glioblastoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (ascites of orb500644 3C2) at 1:2000 overnight at 4C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Pancreas, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with S100B Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
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Sample: Lane 1: Cerebral cortex (Rat) Lysate at 40 ug, Lane 2: Spinal cord (Mouse) Lysate at 40 ug, Lane 3: Spinal cord (Rat) Lysate at 40 ug, Lane 4: Cerebellum (Mouse) Lysate at 40 ug, Lane 5: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-S100B (orb500644) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 11 kD, Observed band size: 9 kD. |
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IHC-P analysis of mouse spinal cord using S100B Monoclonal Antibody at 1:2000 dilution. |
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IHC-P analysis of rat spinal cord using S100B Monoclonal Antibody at 1:2000 dilution. |
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