SLC22A17 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB500631
Artikelname: SLC22A17 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB500631
Hersteller Artikelnummer: orb500631
Alternativnummer: BYT-ORB500631-50,BYT-ORB500631-100,BYT-ORB500631-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human SLC22A17 (211-300/538aa)
Konjugation: Unconjugated
Alternative Synonym: 24p3R, BOCT, BOIT, NGALR, NGALR2, NGALR3, hBOIT, rBOCT, S22AH_HUMAN, SLC22A17, 24p3 receptor (24p3R), Brain-type organic cation transporter, Lipocalin-2 receptor, Neutrophil gelatinase-associated lipocalin receptor (NgalR), S22AH_RAT,
SLC22A17 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 58 kDa
Pubmed: 41386104
UniProt: Q8WUG5
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SLC22A17
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (orb500631) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (orb500631) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (orb500631) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: U2os (Human) Cell Lysate at 30 ug, Lane 3: U87MG (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Lane 5: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-SLC22A17 (orb500631) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
IHC-P analysis of rat cerebellum using SLC22A17 Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse brain tissue using SLC22A17 Polyclonal Antibody at 1:400 dilution.