Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (orb500631) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (orb500631) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC22A17) Polyclonal Antibody, Unconjugated (orb500631) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 2: U2os (Human) Cell Lysate at 30 ug, Lane 3: U87MG (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Lane 5: Siha (Human) Cell Lysate at 30 ug, Primary: Anti-SLC22A17 (orb500631) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
IHC-P analysis of rat cerebellum using SLC22A17 Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse brain tissue using SLC22A17 Polyclonal Antibody at 1:400 dilution.
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