Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cystatin-C) Polyclonal Antibody, Unconjugated (orb500486) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CST3) Polyclonal Antibody, Unconjugated (orb500486) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: MDA-MB-231 (Human) Cell Lysate at 30 ug, Primary: Anti-Cystatin-C (orb500486) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13 kD, Observed band size: 13 kD.
Sample: Testis (Mouse) Lysate at 40 ug, Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Cystatin-C (orb500486) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13 kD, Observed band size: 13 kD.
IHC-P analysis of rat kidney using CST3 Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of Human kidney using Cystatin-C Polyclonal Antibody at 1:200 dilution.
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