4% Paraformaldehyde-fixed MCF-7 (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (PTEN) monoclonal Antibody, unconjugated (orb500350) 1:100, 90 min at 37C, followed by conjugated Goat Anti-Mouse IgG antibody (green, orb868805) at 37C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTEN) Polyclonal Antibody, Unconjugated (orb500350) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTEN) Polyclonal Antibody, Unconjugated (orb500350) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PTEN) Polyclonal Antibody, Unconjugated (orb500350) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
IHC-P analysis of rat kidney using PTEN Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of mouse kidney using PTEN Polyclonal Antibody at 1:200 dilution.
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