Topoisomerase I Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB499925
- Bilder (6)
| Artikelname: | Topoisomerase I Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB499925 |
| Hersteller Artikelnummer: | orb499925 |
| Alternativnummer: | BYT-ORB499925-50,BYT-ORB499925-100,BYT-ORB499925-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Topoisomerase I (401-500/765aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | TOPI, D130064I21Rik, Top-1, Ab2-086, TOP1_HUMAN, TOP1, DNA topoisomerase I, 5.6.2.1, TOP1_MOUSE, TOP1_RAT, |
| Topoisomerase I Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 91 kDa |
| UniProt: | P11387 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | TOP1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Topoisomerase I) Polyclonal Antibody, Unconjugated (orb499925) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human LO2 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human SW480 cell lysates, Primary: Anti-Topoisomerase I (orb499925) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 91 kDa, Observed band size: 100 kDa. |
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