Bcl-2 Mouse Monoclonal Antibody, Clone: [8B5], Unconjugated
Artikelnummer:
BYT-ORB499777
- Bilder (9)
| Artikelname: | Bcl-2 Mouse Monoclonal Antibody, Clone: [8B5], Unconjugated |
| Artikelnummer: | BYT-ORB499777 |
| Hersteller Artikelnummer: | orb499777 |
| Alternativnummer: | BYT-ORB499777-50,BYT-ORB499777-100,BYT-ORB499777-200 |
| Hersteller: | Biorbyt |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Bcl-2 (51-150/239aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Bcl-2, PPP1R50, BCL2_HUMAN, BCL2, |
| Bcl-2 Mouse Monoclonal Antibody |
| Klonalität: | Monoclonal |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [8B5] |
| Molekulargewicht: | 26 kDa |
| UniProt: | P10415 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | BCL2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Bcl-2) Monoclonal Antibody, Unconjugated (orb499777) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: Jurkat (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-Bcl-2 (orb499777) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 26 kD. |
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Sample: LOVO (Human) Cell Lysate at 30 ug, Primary: Anti-Bcl-2 (orb499777) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 26 kD. |
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Sample: LOVO Cell (Human) Lysate at 40 ug, MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-Bcl-2 (orb499777) at 1/2 000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 28 kD. |
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IHC-P analysis of human colon carcinoma using Bcl-2 Polyclonal Antibody at 1:200 dilution. |
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IHC-P analysis of rat kidney using Bcl-2 Monoclonal Antibody at 1:200 dilution. |
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