Phospho-p107 (Ser975) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB4607
Artikelname: Phospho-p107 (Ser975) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB4607
Hersteller Artikelnummer: orb4607
Alternativnummer: BYT-ORB4607-50,BYT-ORB4607-100,BYT-ORB4607-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Ser975 PG(P-S)PR
Konjugation: Unconjugated
Alternative Synonym: RBL1 | p107 (p-S975), p-p107, phospho-p107, CP107, PRB1, p107, RBL1_HUMAN, RBL1, 107 kDa retinoblastoma-associated protein (p107), RBL1_MOUSE,
Phospho-p107 (Ser975) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 121 kDa
UniProt: P28749
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: RBL1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Anwendungsbeschreibung: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p107 (Ser975)) Polyclonal Antibody, Unconjugated (orb4607) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p107 (Ser975)) Polyclonal Antibody, Unconjugated (orb4607) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p107 (Ser975)) Polyclonal Antibody, Unconjugated (orb4607) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: K562 Cell (Human) Lysate at 40 ug, Primary: Anti-phospho-p107 (Ser975) (orb4607) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 121 kDa.
Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HL-60 cell lysates, Primary: Anti-phospho-p107 (Ser975) (orb4607) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 130 kDa.
IHC-P analysis of rat lung using phospho-p107 Ser975 Polyclonal Antibody at 1:200 dilution.
Western blot:K562 Cell(Human),Primary:Anti-phospho-p107(Ser975) at 1:300 dilution.



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