Phospho-ATF2 (Ser322) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB4589
Artikelname: Phospho-ATF2 (Ser322) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB4589
Hersteller Artikelnummer: orb4589
Alternativnummer: BYT-ORB4589-50,BYT-ORB4589-100,BYT-ORB4589-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse, Rat
Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser322 NT(p-S)GR
Konjugation: Unconjugated
Alternative Synonym: ATF2 (p-S322), p-ATF2, phospho-ATF2, CRE-BP1, CREB-2, CREB2, HB16, TREB7, Atf-2, CRE-BP, D130078H02Rik, Tg(Gzma-Klra1)7Wum, mXBP, ATF2_HUMAN, ATF2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2 (CREB-2 | cAMP-responsive element-binding protein 2), cAMP response element-binding protein CRE-BP1, CREBP1, ATF2_MOUSE, MXBP protein, ATF2_RAT,
Phospho-ATF2 (Ser322) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 55 kDa
UniProt: P16951
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: ATF2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Anwendungsbeschreibung: Modification: Phosphorylated
Western blot analysis of Liver (Mouse) Lysate using ATF2 (phospho-Ser322) antibody.
Western blot analysis of Pancreas (Mouse) Lysate using ATF2 (phospho-Ser322) antibody.
Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-ATF2 (Ser322)) Polyclonal Antibody, Unconjugated (orb4589) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-p-ATF2 (Ser322) (orb4589) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.
Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-p-ATF2 (Ser322) (orb4589) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.
Sample: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-p-ATF2 (Ser322) (orb4589) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.