TM7SF2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB4574
Artikelname: TM7SF2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB4574
Hersteller Artikelnummer: orb4574
Alternativnummer: BYT-ORB4574-50,BYT-ORB4574-100,BYT-ORB4574-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human TM7SF2/DHCR14A (51-150/418aa)
Konjugation: Unconjugated
Alternative Synonym: ANG1, C14SR, DHCR14A, NET47, 3110041O18Rik, Dhcr14, ERG24_HUMAN, TM7SF2, Delta-14-SR, 3-beta-hydroxysterol Delta (14)-reductase, Another new gene 1 protein, C-14 sterol reductase (C14SR), Putative sterol reductase SR-1, Sterol C14-reductase, Transmembrane 7 superfamily member 2, 1.3.1.70, ERG24_MOUSE,
TM7SF2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 46 kDa
Pubmed: 34667152
UniProt: O76062
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: TM7SF2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Human adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TM7SF2) Polyclonal Antibody, Unconjugated (orb4574) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TM7SF2) Polyclonal Antibody, Unconjugated (orb4574) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human prostatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TM7SF2) Polyclonal Antibody, Unconjugated (orb4574) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Rat Adrenal gland tissue lysates, Primary: Anti-TM7SF2 (orb4574) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 58 kDa.
Paraformal