Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (orb4461) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (orb4461) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-CTNNBIP1 (orb4461) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 9 kD, Observed band size: 9 kD.
WB analysis of human U937 cell lysate at 30 ug using CTNNBIP1 antibody
IHC-P image of mouse brain tissue using CTNNBIP1 antibody
IHC-P image of mouse brain tissue using CTNNBIP1 antibody
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