The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.
Reinheit:
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Formulierung:
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequenz:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser
Anwendungsbeschreibung:
Solubility: It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of D10S cells is < 0.03 ng/ml. Application Notes: Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard
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