Angiotensin II Type 1 Receptor Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB4245
- Bilder (7)
| Artikelname: | Angiotensin II Type 1 Receptor Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB4245 |
| Hersteller Artikelnummer: | orb4245 |
| Alternativnummer: | BYT-ORB4245-50,BYT-ORB4245-100,BYT-ORB4245-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human AT1R (151-250/359aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R, 1810074K20Rik, AT1a, AT2R1A, Agt1ar, Agtr-1a, Agtr1, Angtr-1a, AT2R1B, Agtr-1b, Angtr-1b, AT3, AT1R, AGTR1_HUMAN, Angiotensin II type-1 receptor (AT1 receptor), AGTR1A, AGTRA_MOUSE, Angiotensin II type-1 receptor A (AT1 receptor A), AGTRB_MOUSE, Angiotensin II type-1 receptor B (AT1 receptor B), AGTRA_RAT, AGTRB_RAT, |
| Angiotensin II Type 1 Receptor Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 39 kDa |
| UniProt: | P30556 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | AGTR1 |
| Application Verdünnung: | WB (Human, Mouse): 1:500-2000 IHC-P/IHC-F (Human, Mouse, Rat): 1:100-500 IF (Human, Mouse, Rat): 1:100-500 |
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orb4245 |
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IHC-P of rat pancreas tissue (AT1R antibody at 1:200) |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (orb4245) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (orb4245) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: HepG2 (human) cell Lysate at 40 ug, Primary: Anti-Angiotensin II Type 1 Receptor (orb4245) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 49kD. |
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Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-Angiotensin II Type 1 Receptor (orb4245) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 36kD. |
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Tissue/Cell: human pneumonia tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated (orb4245) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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