Human TNF-alpha Protein, His Tag, Unconjugated

Artikelnummer: BYT-ORB383503
Artikelname: Human TNF-alpha Protein, His Tag, Unconjugated
Artikelnummer: BYT-ORB383503
Hersteller Artikelnummer: orb383503
Alternativnummer: BYT-ORB383503-100,BYT-ORB383503-1
Hersteller: Biorbyt
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human
Konjugation: Unconjugated
Alternative Synonym: DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFalpha
Human TNF-alpha Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession NP_000585.2).
Molekulargewicht: 18.3 kDa
NCBI: 000585
Puffer: PBS, pH 7.4
Reinheit: >95% as determined by SDS-PAGE and SEC-MALS.
Formulierung: Lyophilized from 0.22 µm filtered solution in PBS, pH7.4 with trehalose as protectant.
Target-Kategorie: TNF-alpha
Anwendungsbeschreibung: Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag (Cat. No. orb383503) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
SDS-PAGE analysis of Human TNF-alpha protein