NRBP2 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB221832
Artikelname: NRBP2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB221832
Hersteller Artikelnummer: orb221832
Alternativnummer: BYT-ORB221832-50,BYT-ORB221832-100,BYT-ORB221832-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human NRBP2 (401-501/501aa)
Konjugation: Unconjugated
Alternative Synonym: TRG16, pp9320, NRBP2_HUMAN, NRBP2, Transformation-related gene 16 protein (TRG-16), NRBP2_MOUSE,
NRBP2 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 58 kDa
UniProt: Q9NSY0
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: NRBP2
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Rat stomach tissue using NRBP2 antibody.
Immunohistochemical staining of human colon cancer tissue using NRBP2 antibody.
Sample: brain (mouse) Lysate at 40 ug, Primary: Anti-NRBP2 (orb221832) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
Sample: Cerebellum (mouse) Lysate at 40 ug, Primary: Anti-NRBP2 (orb221832) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 58 kD, Observed band size: 58 kD.
Tissue/Cell: Human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated (orb221832) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.
Tissue/Cell: Rat stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated (orb221832) 1:400, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.