FAM13C1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB2213
Artikelname: FAM13C1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB2213
Hersteller Artikelnummer: orb2213
Alternativnummer: BYT-ORB2213-50,BYT-ORB2213-100,BYT-ORB2213-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human FAM13C1 (51-150/585aa)
Konjugation: Unconjugated
Alternative Synonym: FA13C_HUMAN, FAM13C, FAM13C1,
FAM13C1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 66 kDa
UniProt: Q8NE31
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: FAM13C
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using FAM13C1 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FAM13C1) Polyclonal Antibody, Unconjugated (orb2213) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FAM13C1) Polyclonal Antibody, Unconjugated (orb2213) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Hela Cell (Human) Lysate at 30 ug, Primary: Anti-FAM13C1 (orb2213) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kD, Observed band size: 63 kD.
Sample: MCF-7 Cell (Human) Lysate at 40 ug, Primary: Anti-FAM13C1 (orb2213) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 66 kD, Observed band size: 66 kD.
Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FAM13C1 Polyclonal Antibody, Unconjugated (orb2213) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.



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