Phospholipase C beta 2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB2211
- Bilder (8)
| Artikelname: | Phospholipase C beta 2 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB2211 |
| Hersteller Artikelnummer: | orb2211 |
| Alternativnummer: | BYT-ORB2211-50,BYT-ORB2211-100,BYT-ORB2211-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human Phospholipase C beta 2 (401-500/1185aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PLC-beta-2, B230205M18Rik, B230399N12, PLCB2_HUMAN, PLCB2, Phosphoinositide phospholipase C-beta-2, Phospholipase C-beta-2 (PLC-beta-2), 3.1.4.11, PLCB2_MOUSE, PLCB2_RAT, |
| Phospholipase C beta 2 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 130 kDa |
| UniProt: | Q00722 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PLCB2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control: Hela Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospholipase C beta 2/AF647 Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions. |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (orb2211) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (orb2211) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PLCB2) Polyclonal Antibody, Unconjugated (orb2211) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospholipase C beta 2) Polyclonal Antibody, Unconjugated (orb2211) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospholipase C beta 2) Polyclonal Antibody, Unconjugated (orb2211) at 1:400 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining. |
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Sample: HL-60 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospholipase C beta 2 (orb2211) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD. |
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Sample: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospholipase C beta 2 (orb2211) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD. |
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