Rat Pituitary Adenylate Cyclase Activating Polypeptide 38 (PACAP-38) ELISA Kit
Artikelnummer:
BYT-ORB1946798
Hersteller Artikelnummer:
orb1946798
Alternativnummer:
BYT-ORB1946798-48,BYT-ORB1946798-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Spezies Reaktivität:
Rat
Alternative Synonym:
PACAP-38
Rat Pituitary Adenylate Cyclase Activating Polypeptide 38 (PACAP-38) ELISA Kit
Detektionsbereich:
7.82-500pg/mL
Sensitivitaet:
4.69 pg/mL
Proben:
serum, plasma, Tissue homogenate and Other biological samples
Target-Kategorie:
PACAP-38
Anwendungsbeschreibung:
Application Notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat PACAP-38. During the reaction, Rat PACAP-38 in the sample or standard competes with a fixed amount of Rat PACAP-38 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-38. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Rat PACAP-38 in tested samples can be calculated by comparing the OD of the samples to the standard curve
orb1946798
orb1946798
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