Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Target-Kategorie:
THB
Anwendungsbeschreibung:
Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrobiopterin(THB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrobiopterin(THB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Tetrahydrobiopterin(THB) in the samples is then determined by comparing the OD of the samples to the standard curve
orb1946757
orb1946757
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
