Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Verdünnung:
WB - 1:500-1:1000
REG3G Antibody (Center) western blot analysis in SH-SY5Y cell line lysates (35 ug/lane). This demonstrates the REG3G antibody detected the REG3G protein (arrow).
Western blot analysis of lysate from SH-SY5Y cell line, using REG3G Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
Anti-REG3G Antibody (Center) at 1:400 dilution + human kidney lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-REG3G Antibody (Center) at 1:1000 dilution + recombinant protein lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-REG3G Antibody (Center) at 1:1000 dilution. Lane 1: mouse small intestine tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 20kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-REG3G Antibody (Center) at 1:2000 dilution. Lane 1: human kidney lysates. Lane 2: human lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-REG3G Antibody (Center) at 1:100 dilution. Lane 1: human kidney lysates. Lane 2: human pancreas lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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