Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Verdünnung:
FC - 1:25, WB - 1:1000
Overlay histogram showing HepG2 cells stained (green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37C. Isotype control antibody (blue line) was rabbit IgG1 (1 µg/1x10 6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Western blot analysis of ABCB5 Antibody (N-term) in A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab.
Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.
Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.
Anti-ABCB5 Antibody (N-term) at 1:2000 dilution + Mouse eyeball lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ABCB5 Antibody (N-term) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-ABCB5 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: A375 whole cell lysate. Lane 5: A2058 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
