Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Verdünnung:
WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50
Fluorescent confocal image of U251 cell stained with p53 Antibody (S315).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with p53 primary antibody (1:25, 1 h at 37C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). p53 immunoreactivity is localized to Nucleus significantly.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with p53 Antibody (S315), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.
p53 Antibody (S315) western blot analysis in Daudi cell line lysates (35 ug/lane). This demonstrates the p53 antibody detected the p53 protein (arrow).
All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-p53 Antibody (S315) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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