Goat anti-CD32 / FCGR2B Antibody, Unconjugated, Polyclonal

Artikelname:	Goat anti-CD32 / FCGR2B Antibody, Unconjugated, Polyclonal
Artikelnummer:	BYT-ORB19010
Hersteller Artikelnummer:	orb19010
Alternativnummer:	BYT-ORB19010-100
Hersteller:	Biorbyt
Wirt:	Goat
Kategorie:	Antikörper
Applikation:	ELISA, FC, IF, IHC, WB
Spezies Reaktivität:	Human
Konjugation:	Unconjugated
Alternative Synonym:	anti-CD32 antibody, anti- CD32 antigen antibody, anti- CD32B antibody, anti- CDw32 antibody, anti- Fc fragment of IgG, low affinity II, receptor for (CD32) antibody, anti- Fc fragment of IgG, low affinity IIb, receptor antibody, anti- Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody, anti- Fc fragment of IgG, low affinity IIb, receptor for (CD32) antibody, anti- Fc fragment of IgG, low affinity IIb, receptor for antibody, anti- Fc gamma receptor IIB antibody, anti- Fc gamma RII antibody, anti- Fc gamma RIIB antibody, anti- Fc-gamma RII antibody, anti- Fc-gamma RII-b antibody, anti- Fc-gamma-RIIb antibody, anti- FCG2 antibody, anti- FCG2B_human antibody, anti- Fcgr2 antibody, anti- FCGR2B antibody, anti- FCRII antibody, anti- FcRII-b antibody, anti- IGFR2 antibody, anti- IgG Fc receptor II beta antibody, anti- IgG Fc receptor II-b antibody, anti- Low affinity immunoglobulin gamma Fc region receptor II antibody, anti- Low affinity immunoglobulin gamma Fc region receptor II-b antibody, anti- Ly-17 antibody, anti- Lym 1 antibody, anti- Lymphocyte antigen 17 antibody

Goat polyclonal antibody to CD32b

Klonalität:	Polyclonal
Molekulargewicht:	34.0, 31.9, 31.9, 34.0, 33.4
Puffer:	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequenz:	PDALEEPDDQNRI
Target-Kategorie:	CD32 / FCGR2B

Application Verdünnung:

ELISA: 1:32000, WB: 0.3-1 µg/ml

Anwendungsbeschreibung:

Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 34kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0kDa according to NP_003992.3,). In transfected HEK293 transiently expressing CD32 a band of approx. 40kDa (glycoprotein) is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/ml

	1 µg/ml staining of Daudi cell lysate (RIPA buffer, 35 µg protein in RIPA buffer). Detected by chemiluminescence.
	8 µg/ml staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
	Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
	Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor 488 secondary antibody (2 µg/ml).
	0.5 µg/ml staining of Human Placenta lysate (RIPA buffer, 35 µg protein in RIPA buffer). Detected by chemiluminescence.
	Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488 secondary antibody (1 µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.