RPS6KA1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB185890
Artikelname: RPS6KA1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB185890
Hersteller Artikelnummer: orb185890
Alternativnummer: BYT-ORB185890-50,BYT-ORB185890-100,BYT-ORB185890-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IF, IHC-Fr, IHC-P
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human RPS6KA1 (51-150/735aa)
Konjugation: Unconjugated
Alternative Synonym: KS6A1_MOUSE, Rps6ka1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1 | p90RSK1 | p90S6K), MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a | MAPKAP kinase 1a | MAPKAPK-1a), Ribosomal S6 kinase 1 (RSK-1), 2.7.11.1, Mapkapk1a, Rsk1, KS6A1_RAT, HU-1, MAPKAPK1, RSK, p90Rsk,
RPS6KA1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 90 kDa
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: RPS6KA1
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (RPS6KA1) polyclonal Antibody, Unconjugated (orb185890) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (orb185890) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (orb185890) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RPS6KA1) Polyclonal Antibody, Unconjugated (orb185890) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
IHC-P analysis of human colon carcinoma using RPS6KA1 Polyclonal Antibody at 1:400 dilution.
IHC-P analysis of mouse intestine tissue using RPS6KA1 Polyclonal Antibody at 1:400 dilution.



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