MEX3D/RNF193 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB185612
Artikelname: MEX3D/RNF193 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB185612
Hersteller Artikelnummer: orb185612
Alternativnummer: BYT-ORB185612-50,BYT-ORB185612-100,BYT-ORB185612-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human MEX3D/RNF193 (201-300/651aa)
Konjugation: Unconjugated
Alternative Synonym: MEX-3D, MEX3, OK/SW-cl.4, RKHD1, RNF193, TINO, MEX3D_HUMAN, MEX3D, RING finger and KH domain-containing protein 1, RING finger protein 193, KIAA2031,
MEX3D/RNF193 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 65 kDa
UniProt: Q86XN8
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: MEX3D
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
IHC-P image of mouse brain tissue using MEX3D antibody
Immunohistochemical staining of paraffin embedded human brain glioma using MEX3D antibody
Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (orb185612) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (orb185612) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (orb185612) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: U251 Cell (Human) Lysate at 40 ug, Primary: Anti-MEX3D/RNF193 (orb185612) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 62 kD.



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