0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:
Liquid
Target-Kategorie:
RANGRF
Application Verdünnung:
IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of Mouse testis using MOG1 antibody.
Immunohistochemical staining of Mouse brain using MOG1 antibody.
Immunohistochemical staining of Rat testis using MOG1 antibody.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG1) Polyclonal Antibody, Unconjugated (orb185183) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG1) Polyclonal Antibody, Unconjugated (orb185183) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MOG1) Polyclonal Antibody, Unconjugated (orb185183) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
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